Polarimeter Apparatus (Side View)
Analyzer View (Looking into detector)

Concept Quiz

Optical Activity & Biot's Law

Chiral molecules lack an internal plane of symmetry. When plane-polarized light passes through a solution of chiral molecules, the plane of polarization is rotated. This phenomenon is called optical activity.

$$\alpha = [\alpha]_D^T \cdot c \cdot l$$
$\alpha$ = Observed Rotation
$[\alpha]$ = Specific Rotation (constant for a pure enantiomer)
$c$ = Concentration (g/mL)
$l$ = Tube Path Length (dm)